[Efficacy of human umbilical cord mesenchymal stem cells in the treatment of chronic nonbacterial prostatitis in rats].
DU Haiyan H, Zhang Kun K, Zhang Qin Q, Li Linghui L et al.
Objective To investigate the efficacy of human umbilical cord mesenchymal stem cell (hUCMSC) for the treatment of chronic nonbacterial prostatitis (CNP) model rats. Methods Thirty-two male Sprague-Dawley rats were randomized into four experimental subgroups: control, model, tamsulosin, and hUCMSC, with eight animals allocated to each group. CNP models were induced in all subgroups, except the control. After 28 days of modeling, the hUCMSC group was injected weekly with 1×106 cells/kg P5 hUCMSC cell suspension by tail vein for 4 weeks. In the control and model groups, equivalent volumes of normal saline were administered via injection. And the tamsulosin group was given daily gavage of 0.022 mg/kg tamsulosin solution for 28 days. After 4 weeks of treatment, the basic indexes including body weight, intake and water intake, and prostate index were analyzed in each group, HE staining was performed to characterize the histopathological features of the prostate. The levels of serum TNF-α, IL-1β, and IL-10 in rats were detected by ELISA. qRT-PCR was used to detect the mRNA expression of TNF-α and IL-1β in prostate tissues. And Western blot was performed to detect the protein expression of the NF-κB inflammatory pathway. Results At the end of treatment, compared with the model group, the body weight and water intake of the rats in the tamsulosin group and hUCMSC group increased significantly (P<0.05). There was a significant decline in prostate indexes and prostate wet weight (P<0.01). And interstitial inflammatory cells in prostate tissues were reduced. The levels of serum TNF-α and IL-1β and the expression of TNF-α and IL-1β mRNA in prostate tissues were significantly reduced (P<0.01). An opposite tendency was observed in IL-10 levels (P<0.01). p-IκBα/IκBα and p-NF-κB p65/NF-κB p65 ratios were significantly reduced (P<0.01). Conclusion hUCMSC improve the pathological state of rat prostate, inhibit the overexpression of pro-inflammatory factors, down-regulate the phosphorylation level of NF-κB signaling pathway, and alleviate the inflammatory response in CNP rats.