butocin (butocin / Benin)

Mutagenicity of cytostatic drugs in a bacterial system. I. Ames test.

Marhan J J

A set of nine cytostatic drugs were tested in the standard plate incorporation method of Ames reversion test using four Salmonella typhimurium his strains. Six of them were classical and commonly used cytostatics, three (cloturin, butocin and oracin) are at different stages of development. The results showed 6-mercaptopurine, cloturin, adriamycin, mitoxantron, cyclophosphamide and lomustine to be mutagenic; butocin, oracin and tris(2-chloroethyl)amine were negative in this test.

Mutagenicity of cytostatic drugs in a bacterial system. II. DNA-repair test.

Marhan J J

A liquid micromethod modification of the DNA-repair test using an automatic growth analyzer was developed. The wild strain Escherichia coli WP2 and six repair-deficient isogenic test strains were used. To compare this repair test with the conventional plate Ames test, a set of nine cytostatics were tested. Cloturin, adriamycin, mitoxantron, oracin, lomustine and tris(2-chloroethyl)amine were found to be positive, and 6-mercaptopurine, butocin and cyclophosphamide negative. The experimental micromethod appears to be useful for assessing the differential lethality in bacteria and can be combined in any short-term test system to predict genotoxicity.

[Determination of the tolerance and pharmacokinetics of butocin in persons with progressive polyarthritis].

Buchar E E, Perlík F F, Novotná P P, Janků I I

[Binding of butocin to serum proteins (author's transl)].

Grafnetterová J J, Kalousek I I, Vodrázka Z Z, Smahel O O

The binding of N-[-5-(6-purinylthio)-valeryl]-glycin ethylester (butocin, PVG) to serum proteins and pure human albumin was studied using the method of equilibrium dialysis. Its binding to protein in sera diluted 1:1 of 10 patients with malignant disease averaged 48.4 +/- 7.07%. At the butocin concentration of 20 micrograms/ml an average of 36% of butocin were bound to pure albumin. Only a small portion was bound to globulin fractions. Measurements of the saturation curve showed butocin to be bound to albumin molecule by one binding centre with a microscopic association constant kappa = 1.7 . 10(3) mol/l.